《植物生理学报》 2010, 46(9): 943-948

HCO₃^-、K⁺和 HSO₃^-对黄瓜幼苗光合作用的影响

郝建军*,**, 陈嫚*, 于洋

沈阳农业大学生物科学技术学院, 沈阳110161

通信作者：郝建军；E-mail: haojianjun106@126.com；Tel: 024-88487764

摘　要：

本试验以‘ 津春4 号’ 黄瓜幼苗叶片为试材, 研究HCO3-、K+ 和HSO3- 对黄瓜幼苗光合作用的影响, 试图用KHCO3 水溶液中的HCO3- 作为碳源来补充CO2 的不足, 同时用NaHSO3 适当的抑制黄瓜的光呼吸,进而提高光合速率。结果表明:HCO3- 可以作为碳源来补充大气中CO2 的不足, HCO3-、K+ 和HSO3- 可以提高光合速率、叶片可溶性糖含量, 可提高叶绿素a 含量、叶绿素b 含量及叶绿素总含量, 从而增强了光合作用的原初反应, 能显著提高PSI 和PSII的光合电子传递速率,提高ATP 合酶的活性, 从而加快了光合磷酸化的进程。通过提高Rubisco 羧化活性、PEPC 酶的含量及活性, 降低Rubisco加氧活性, 加快了CO2的固定与还原。

关键词：HCO3 ; K ; HSO3 ; 黄瓜幼苗叶片; 光合作用

收稿：2010-05-06   修定：2010-07-17

资助：辽宁省教育厅科学技术研究项目(2004A011)。

Effects of HCO₃^-, K⁺ and HSO₃^- on Photosynthesis of Cucumber Seedlings

HAO Jian-Jun*,**, CHEN Man*, YU Yang

College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110161, China

Corresponding author: HAO Jian-Jun; E-mail: haojianjun106@126.com; Tel: 024-88487764

Abstract:

Effects of HCO₃^-, K⁺ and HSO₃^- on photosynthesis of cucumber seedlings was studied in this research. It is tried that HCO₃^- in KHCO₃ be used as a carbon source to supplement the shortage of CO₂. At the same time, NaHSO₃ was used to restrain photorespiration to increase photosynthetic rate of cucumber seedlings. The results showed that HCO₃^- can serve as a carbon source to supplement the lack of tmospheric CO₂. HCO₃^-, K⁺and HSO₃^- can promote photosynthetic rate, raise content of soluble sugar, improve the contents of chlorophyll a, b and total chlorophyll content in the leaves, and thus enhance the initial response of photosynthesis. HCO₃^-,K⁺ and HSO₃^- can significantly improve the electron transport activity of PSI and PSII, enhance ATP enzyme activity and photosynthetic electron transfer rate. HCO₃^-, K⁺ and HSO₃^- enhance RuBPCase activity, and PEPCase activity and content, and debase RuBPOase activity and speed up the fixation and reduction of CO₂.

Key words: HCO₃^-; K⁺; HSO₃^-; cucumber seedlings; photosynthesis